The One that (almost) Got Away

My Muskokan Mystery Mushroom

lots of yellow liquid around the base

Here is a curious individual which escaped identification on the Cain Foray in Muskoka last weekend. Anyone out there have a theory? The experts said it was too difficult to ID because of a lack of info as it was not mature enough, and as I had not retrieved the base, it was unclear whether it was growing on the wood or debris.

It was found growing horizontally out of the side of a hollow tree gall or burl about the size of a cantaloupe on the side of a sugar maple. The 6″ diameter tree had three such large growths, only one of which had an opening. I was unable to retrieve the base, but the firmness of its attachment suggested it was growing on the wood and not on an accumulation of plant matter, or an insect, inside the hollow. It is interesting to theorize on the mushroom’s relation to the gall.

  1. Was the fungus the  cause of the growth
  2. Was it parasitizing the insect or bacteria that caused the growth?
  3. Attacking the tree in a vulnerable location?
  4. Growing on debris accumulated inside the gall?

There was a drop of blood red exudate near the tip, and lots of yellow exudate around the bottom half of the stem. I don’t recall it having a distinct smell or not.

I found it late on the first the day, hence the dark pictures. The poor condition of the specimen on arrival at the ID table, and these crappy pictures taken with my iPhone and a flashlight, were not much help. My first indication that as a newbie, I had come ill-prepared for the foray.

Late Breaking Update Sept 22: Thanks to Jan Thornhill of Mycological Society of Toronto on the FaceBook Mushroom Identification Forum for solving the mystery and the correct answer above was # 3. See our discussion below:


Jan Thornhill I think it was a baby Lentils levis – it certainly yellowed as it dried on the table.

Mycognostic Thanks Jan. Looks like you got spell checked there. I’m checking out the Lentinus levis looking for “Baby Pictures”

Jan Thornhill Definitely not lentils! I’m on laptop and spellcheck is still on.

Mycognostic Well it definitely was tenacious and it sure meets a lot of Lentinus levis’ characteristics in terms of habit and location, but no mention of secretions.

But if it was basically still in primordial form (as it was still in the low light/high CO2 environment of the burl), it was in its pre-cap stage and sweating exudate which isn’t mentioned when describing the mature mushroom. That’s something I’ve seen in cultivated mushroom of various species.

http://www.messiah.edu/…/species…/Lentinus%20levis.htm
Lentinus levis
Scientific name: Lentinus levis (Berk. & M.A. Curtis) MurrillDerivation of name: Lentus means “pliable” and “tenacious” as in chewy. Levis means polished or smooth. Synonyms: Pleurotus levis (Berk. & M.A. Curtis) Singer; Panus strigosus Berk. & M.A. Curtis; Panuslevis Berk. & M.A. Curtis Common na…
messiah.edu

a picture of a G. lucidum primordium secreting a fluid that is usually not mentioned in describing the mature fruit body

Mycognostic Thanks again Jan. I haven’t found any pics of primordial L. levis but that’s not surprising. I have attached a picture of a G. lucidum primordium secreting a fluid that is usually not mentioned in describing the mature fruit body.

Jan Thornhill Guttation/exudiate is hardly ever mentioned, even when it is diagnostic, i.e. for Inonotus glomeratus that bleeds pretty spectacular black tarry drops.

The 2016 Cain Foray

img_9661It was very exciting to be the least knowledgeable of 40 or so mushroom lovers on the 39th Annual Cain Foray. Apparently, between us we collected and identified over 250 different fungi. Thank you to the Mycological Society of Toronto for being so welcoming and generous with your knowledge.

It was amazing to watch the many amateur and professional mycologists Identifying the mushrooms as they came in. The special guest was Walter Sturgeon, the author of Mushrooms of the Northeast: A Simple Guide to Common Mushrooms and many articles on mycology. I won a signed copy of his book!

On Saturday night Chefs Dwayne and Garrett and their team cooked up a tasting table with Chicken of the Woods, Chanterelles, Boletes, and Tempura battered Aborted Entoloma (more appetizingly known as the Shrimp of the Woods). Absolutely delicious, thanks guys.

I will return to the Cain Foray next year for sure, and I will have to up my game.
a) I need to bring a proper basket. The knapsack full of LCBO bags doesn’t cut it and marked me as a total nube. Plus many of my more delicate finds (including Destroying Angel, Amanita bisporigera!) were destroyed. Well, I guess it serves her right.
b) I can’t rely on the iPhone to take decent pictures and sometimes information from those pics can be crucial to identifying a specimen. Plus they will be much nicer for the blog. Next time I’ll bring the Canon and some flat panel LED lights.
c) It’s time to brush up on my Latin and start learning the scientific names of fungi other than the ones I cultivate. These cats are dead serious about identification of all fungi. The most daunting thing about the learning curve is that the experts say the Latin names are constantly changing now (partially due to DNA testing I assume). Hopefully I’ll learn the right ones first. I’ve ordered Mushrooms Demystified on Weird and Wonderful Wild Jan Thornhill’s  suggestion, I hope the newest edition is up to date.

Below is a gallery of pics from the Foray. I’ll just throw them up for now and hopefully get around to labeling them someday.

Taming the Wild Chicken of the Woods

Finding wild mushrooms, then cloning and domesticating them is one of the most fun and challenging mycological exercises for me. I have been successful cloning wild Turkey Tail, as well as King Oysters from the store, today I’m trying to tame a wild Chicken of the Woods. This one appears every year about this time. It seems to be higher up the tree each year. This year it was too high to get with the ladder so I brought an arborists’ pole saw.

A fresh part of the growing edge was put into Hydrogen Peroxide. (H2O2) to clean the outside, and then transferred to the prepped glove box. Because I’m cloning to nutrifed agar I must use sterile techniques. Gloves and Tyvek sleeves misted with isopropyl alcohol keep your germs out of the clean space. The blade is flame sterilized (careful with the alcohol around). The mushroom is torn open to prevent the blade transferring contaminants from the surface. A small piece is deftly transferred to the plate. The plate is sealed with ParaFilm or a homemade equivalent. Plates are labeled and incubated. After you are finished clonong, seal the petri dishes. Instead of ParaFilm I make my own sealing tape with short widths cut from a roll of cellophane.

Five days later, after returning from the Cain Foray (where we ate the Chicken, along with many other fungal delights cooked up by Chefs  Dwayne and Garrett) to find a pretty good results. All of the plates show growth in all directions (if perhaps a bit thin), and there doesn’t seem to be any contamination.

Stay tuned for more…

Cultivating Corn Smut (Ustilago maydis) Part 1

“corn smut” (Ustilago maydis), huitlacocheI won’t be able to continue this experiment until next year as it will involve growing corn and inoculating it with spores of the Ustilago maydis I was given by my neighbor Justine. For now I’ll put up some pictures of the original infected corn cob and of the process of collecting and storing the spores.

In the mean time I can study this paper: Production of huitlacoche, Ustilago maydis: timing inoculation and controlling pollination to get ready for growing season.

Here are some pics for now. Here is Part 2 of the experiment.

This Smut Has Some Redeeming Qualities!

Making Grain Spawn Using Wild Bird Seed

Cultivating Shiitake on Oak Logs

My Indoor Cultivation Set-up

Incubator, glove box and sink in a corner of the basement I have my “lab”, incubation space and fruiting chamber down in the basement. It’s a pretty simple set-up and I feel I am about to outgrow it, but here are some pics of the current state of it. If I make improvements I post them.

The lab where i do my sterile work (ie. mostly stuff involving petri dishes with agar) is in a corner of the basement to avoid air currents. The space was originally set-up as a home brewing/wine making space, so there is a sink with a bottle washer! I use a still air box or “glove box” to make the transfers. The box was built by a good friend and fellow mushroom lover. Using a popular design, there are lots of variations, some people use a large clear tub.

In this case, petri dishes with nutrified agar, butane torch, needles and syringes, alcohol spritzer, scalpel and tweezers.I added an electrical outlet and florescent light. I’ve thought about putting a strong UV light in there to sterilize the box between uses, I’ll have to look into the specs for that. Ultimately I will build a Laminar Flow Hood for this kind of work. There are lots of plans out there. Put your glovebox at the right height, mine is too low and I have to bend over to reach the back of the box. Some day I will raise it 6 or 8 inches or so .

The light is turned on first, then the box is cleaned and sprayed with bleach solution. Everything needed for the procedure is wiped down with alcohol and put in the box. In this case, petri dishes with nutrified agar, butane torch, needles and syringes, alcohol spritzer, scalpel and tweezers. Mist the air in the box again with bleach as you close it. Let it sit for a while to settle.

a pair of tyvek sleeves cut from a paint suit, thumb holes help keep them from sliding up when I put on some fresh glovesThis space is used for cloning mushrooms to agar, and transfers of: spores to agar, myceliated agar to Liquid Culture (LC) media, agar to agar, agar to sterilized grain, colonized grain to sterilized/supplemented bulk substrates like sawdust with wheat bran.

Before working in the box I don a pair of Tyvek sleeves cut from a paint suit, thumb holes help keep them from sliding up when I put on some fresh gloves. The gloves get alcohol spray and wipe. Be extremely careful when using alcohol and flame in the same space. The gloves must be dry before sparking the torch. Never spray down the space with alcohol. Use Pinesol, bleach, or vinegar & hydrogen peroxide.

the incubator, which is in my case just three pieces of plywood making a box on the counter with some shelving a thermostat and heater.The dry gloved hands, fingers together and knuckles first go under the rubber curtains through the holes into the sterile space, other pre-serilized objects or tools can be passed through these holes but it should be avoided if possible, and the lid should not be opened before you are finished.

Planning is key to getting everything ready for the sterile transfers, which must be done as swiftly and efficiently as possible. None of the tools, media, or fungi can touch any surface or they must be re-sterilized. Maybe more on these procedures later.

Inoculated media are put in the incubator, which is in my case just three pieces of plywood making a box on the counter with some shelving a thermostat and heater. The space should be warm, about 28C for most species. the humidity is not curcial as the containers incubated in here are generally sealed. A proper incubation room would probably have humidity control.

a seedling germination tented greenhouse shelf thingie in another part of the basement which I try to keep around 18C. A small humidifier tries to keep the humidity around 95%Lastly we come to the fruiting chamber currently a seedling germination tented greenhouse shelf thingie in another part of the basement which I try to keep around 18C. A small humidifier tries to keep the humidity around +95%

This is the weak link in the chain. It’s hard to maintain the temp/humidity/CO2 content and keep out fungus gnats (yuk). Each mushroom requires different and very specific conditions. Plans are for converting an old shower stall into a more air tight cabinet.

Sometimes, if the weather is just right, it’s better to just to take them to humid shady spot outside and let nature take its course.

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Blue Oyster Growing on Dishwater

Hardy stuff! Here is a picture of Blue Oyster mycelium growing off discarded grain spawn in dirty dish water. The same strain just fruited from a pile of old bulk straw/sawdust/woodchip spawn from last year which survived a winter with -40C temperatures and then a historic drought.

Foto Foray at Temagami